How to Test a Microscope

Microscopes are useful tools for studying objects not visible to the naked eye. Proper care and use of your microscope lessens the need for repair or replacement. Testing the microscope at regular intervals can pinpoint small issues which can be easily fixed. Professionals must fix microscopes with major problems found during such tests. Always follow proper handling and storage instructions when working with a microscope. Keeping a dust cover on the microscope when not in use can prevent any problems caused by dust.

• 1

  Plug the microscope into a reliable electrical source. Using electrical outlets with too much or too little voltage will skew the results of the test and could possibly harm the instrument. Check the light source of the microscope to make sure the light comes on. If there is no light, check to see if the iris mechanism is open.

• 2

  Secure a prepared slide to the specimen stage. Slides with colorful and contrasting dyes make better testing specimens. It will be easier to focus and check resolution later in the test. Check the slide for fingerprints or other debris before putting it on the stage. Dirty slides can cause distorted results.

• 3

  Change the objective to the lowest setting. This will give you a view of the entire specimen area on the slide. Move the slide to the center of the field of view. The specimen should be visible even if it is not yet in focus.

• 4

  Turn the coarse adjustment knob to focus the microscope. If you cannot see the specimen, clean the objective with a soft lens cloth. Refocus the microscope until the specimen is clearly visible. At this point, if the specimen is not sharp, continue with the test.

• 5

  Switch the objective to a higher power. The specimen remains mostly focused if the coarse adjustment knob stays in the same position. If necessary, use the fine adjustment knob to bring the specimen fully into sharp focus. Do not use the coarse adjustment knob when viewing slides under high power. It could scratch the lens or crack the slide.

• 6

  Make a note of any problems encountered during the test. Resolution will decrease as the objective is increased. Large issues may require professional attention. The more information you can provide to the repair service will cut lost productivity time and possibly cost.